On top of that, whether the recognized focus on proteins afflicted by OGT deletion are classified as the vital proteins contributing to a specific pathology is unclear.This step was repeated 4 instances. Protein A/G Magnetic Beads had been additional on the samples, which were being incubated with rotation for 2 h at four °C. The beads had bee
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Considering that its introduction, the propidium iodide (PI) flow cytometric assay has been widely useful for the evaluation of apoptosis in different experimental models. It is predicated within the principle that apoptotic cells, among other normal characteristics, are characterized by DNA fragmentation and, consequently, loss of nuclear DNA cont